Distance learning is an educational format that involves guided independent study without the physical presence of academic staff and students in the classroom. Due to the COVID-19 pandemic, distance learning has become the predominant method of education in the Czech Republic. A research study was conducted using a questionnaire survey with the aim of collecting data from academic staff and students of Czech colleges and universities in order to determine their perception of distance learning. 84 responses from academic staff and 161 responses from students were subjected to statistical testing. The results showed that although students' motivation to study in distance learning was higher, compared to motivation during face-to-face learning, and although they evaluated the level of cooperation with classmates positively, they perceived their work and approach to study as average to below average. While academic staff rated their work and approach to teaching, cooperation with colleagues and provided study materials as excellent, distance learning was not beneficial for them in terms of acquiring new skills and their work motivation was not proven to be higher. Overall, academic staff expressed higher satisfaction with distance learning than students. Surprisingly, the majority of respondents did not look forward to face-to-face learning.